MuSeqBox

MuSeqBox parses BLAST output to extract, filter, and tabulate BLAST hits for sequence annotation and large-scale sequence comparison, including Expressed Sequence Tag (EST) collection analysis.

Key Features:

• Parsing and Tabulation: Systematically processes BLAST (Basic Local Alignment Search Tool) output, extracting essential hit parameters and formatting them into tabular outputs exportable as text or HTML.
• Customizable Filtering: Applies multiple selection criteria to refine BLAST hits based on hit attributes and thresholds.
• Annotation and Database Comparison: Annotates query sequences through simultaneous comparisons against databases using stand-alone or network-client BLAST programs.
• EST Analysis: Supports detailed examination and annotation of Expressed Sequence Tag (EST) collections.

Scientific Applications:

• Genomics: Enables large-scale sequence comparison and annotation in genomic studies.
• Transcriptomics: Facilitates analysis and annotation of transcript-derived sequences such as ESTs.
• Evolutionary Biology: Supports comparative sequence analyses relevant to evolutionary studies.

Methodology:

Parses BLAST output to extract hit parameters, applies selection criteria to filter hits, formats results into tabular outputs exportable as text or HTML, supports comparisons via stand-alone or network-client BLAST programs, and is implemented in standard C++.