MutScreener

MutScreener designs human-specific PCR and sequencing primers to enable mutation screening and high-throughput resequencing of candidate genes and genomic regions.

Key Features:

• Human-Specific Primer Design: Designs primers specific to human gene sequences using public gene annotations and user-provided custom annotations.
• Batch Assay Design: Supports batch processing to design assays for multiple genes or large genomic regions simultaneously.
• Integration with Gene Annotations: Incorporates gene annotation information to align primer placement with structural and functional characteristics of human genes.
• Universal Tagged Primers: Optionally generates universal tagged primers to facilitate high-throughput resequencing workflows.

Scientific Applications:

• Positional cloning and candidate gene strategies: Enables identification of susceptibility genes by facilitating targeted mutation screening in candidate regions.
• Mutation and polymorphism detection: Facilitates pinpointing of mutations or polymorphisms within candidate genes or genomic regions via PCR and sequencing.
• High-throughput resequencing: Supports large-scale resequencing studies through batch assay design and optional universal tagged primers.

Methodology:

Analyzes human gene structures and leverages public or custom gene annotations to design optimal PCR and sequencing primers.