muxQuant

muxQuant performs multiplexed quantitative proteomics by using differential metabolic stable isotope labeling coupled to mass spectrometry to quantify protein abundances across multiple samples.

Key Features:

• Multiplexing Capability: Concurrent analysis of multiple samples using differential metabolic stable isotope labeling to enable comparative quantification across conditions or time points.
• Differential Metabolic Stable Isotope Labeling: Incorporation of isotopically distinct labels into proteins from different samples to differentiate and compare protein abundances by mass-to-charge ratio.
• Mass Spectrometry Integration: Use of mass spectrometry to generate high-resolution mass-to-charge data for identification and quantification of labeled proteins.
• Data Integration and Quantification: Integration of labeled-sample mass spectrometry data to perform precise relative quantification of protein levels across multiplexed samples.

Scientific Applications:

• Studying Stress Responses: Quantitative profiling of the heat-shock response in Arabidopsis thaliana, including confirmation of known heat-shock proteins and discovery of previously uncharacterized proteins associated with heat stress.
• Comparative Proteomics: Comparative analysis of differential protein expression and regulation across multiple biological states, treatments, or time points within a single experimental run.

Methodology:

Data integration and quantification of mass spectrometry measurements using differential metabolic stable isotope labels to enable precise relative comparison of protein abundances across samples.