MyoExplorer

MyoExplorer analyzes single-nucleus RNA sequencing (snRNA-seq) data to characterize nuclear heterogeneity and transcriptional compartmentalization in syncytial skeletal muscle.

Key Features:

• Single-Nucleus Transcriptomics Analysis: Enables examination of isolated nuclei from muscle fibers to identify distinct nuclear subtypes independent of fiber type, including subtypes at neuromuscular and myotendinous junctions.
• Transcriptional Dynamics Analysis: Dissects transcriptional dynamics across nuclear populations to reveal molecular signatures of compartmentalization.
• Disease Model Insights: Analyzes data from the mdx dystrophy mouse to uncover nuclear subtypes associated with muscle repair and necrosis and enables correlation with patient data.
• Functional Compartmentalization: Uses modifications to standard methodologies to resolve compartmentalization within rare and specialized structures such as muscle spindles.
• Sample Contexts: Utilizes snRNA-seq data from uninjured and regenerating muscle tissues to compare nuclear states across physiological and regenerative conditions.

Scientific Applications:

• Muscle Biology Research: Characterizes molecular signatures of myofiber compartments to inform studies of muscle development, regeneration, and disease.
• Translational Medicine: Identifies nuclear populations linked to repair and necrosis in dystrophic muscle that can guide identification of potential therapeutic targets.

Methodology:

Built on single-nucleus RNA sequencing (snRNA-seq) data from uninjured and regenerating muscle tissues and employing computational analyses to dissect nuclear heterogeneity and transcriptional dynamics.