Nannocholopsis gaditana Database

Nannocholopsis gaditana Database provides curated genome assembly, gene annotations, and transcriptomic data for the microalga Nannochloropsis gaditana to support genomic analyses and research on lipid accumulation relevant to biofuel production.

Key Features:

• High-Quality Genomic Assembly: Includes a genome assembly with contigs up to 500 kilobase pairs (kbp) and scaffolds that typically span entire chromosomes.
• Gene Annotation and Characterization: Reports 10,646 complete genes with detailed annotations including characterization of possible alternative transcripts.
• Cellular Process Insights: Highlights genes involved in DNA recombination, RNA silencing, and cell wall synthesis relevant to genetic improvement efforts.
• Transcriptional Profile Analysis: Contains transcriptomic data under nitrogen deficiency showing increased lipid accumulation with largely unchanged expression of lipid biosynthesis genes and pronounced down-regulation of mitochondrial gene expression, indicating reallocation of metabolic resources toward fatty acid synthesis.
• Search and Browsing Capabilities: Provides BLAST search capability and a genome browser for sequence queries and genomic navigation.

Scientific Applications:

• Biofuel Production Optimization: Supports investigation of genetic determinants and pathways to optimize lipid yield for biofuel applications.
• Genetic Engineering and Strain Improvement: Enables identification of candidate genes and processes for genetic modification to improve strain performance.
• Metabolic and Stress-Response Studies: Facilitates analysis of metabolic pathway shifts and transcriptional responses to nitrogen deficiency.

Methodology:

Genome assembly producing large contigs and chromosome-scale scaffolds; gene prediction identifying 10,646 complete genes with annotation of possible alternative transcripts; transcriptomic profiling under nitrogen deficiency; and provision of BLAST search and a genome browser.