Nanopore Current Events Magnifier (nanoCEM)

Nanopore Current Events Magnifier (nanoCEM) facilitates the identification of DNA/RNA modification sites through enhanced visualization and statistical analysis of nanopore sequencing data.

Key Features:

• Multi-method compatibility: Supports four preprocessing methods including 'f5c resquiggle', 'f5c eventalign', 'Tombo' and 'move table'.
• Rescaling techniques: Utilizes rescaling to provide more accurate and comparable visualization of current events.
• Statistical feature calculation: Calculates various statistical features to enable comparison between samples and showcase modified sites.
• Cross-platform applicability: Applicable to RNA and DNA analysis across multiple flow cell types.

Scientific Applications:

• DNA modification site identification: Enables detection and visualization of base modifications in DNA molecules via electrical current analysis.
• RNA modification site identification: Enables detection and visualization of base modifications in RNA molecules via electrical current analysis.
• Sample comparison: Allows researchers to effectively observe differences between samples at modification sites.

Methodology:

Utilizes rescaling techniques and calculates various statistical features to provide accurate and comparable visualization of current events.