NanoCoV19

NanoCoV19 performs rapid detection and lineage identification of SARS-CoV-2 from nanopore sequencing technology (NST) data using phylogenetic-tree and hotspot mutation analyses to support genetic surveillance.

Key Features:

• Rapid Detection: Completes SARS-CoV-2 detection from nanopore sequencing technology (NST) data within one hour, enabling faster turnaround than Illumina-based workflows.
• Lineage Identification: Distinguishes and traces SARS-CoV-2 lineages including alpha, beta, delta, gamma, lambda, and omicron.
• Integration of Phylogenetic Analysis: Incorporates phylogenetic-tree analysis to infer evolutionary relationships among viral strains.
• Hotspot Mutation Analysis: Identifies hotspot mutations in SARS-CoV-2 genomes to pinpoint critical genetic changes.

Scientific Applications:

• Rapid Subtyping: Provides fast subtyping of SARS-CoV-2 sequences for outbreak investigation.
• Lineage Surveillance: Enables tracking of variant spread and emergence through lineage assignment.
• Public-Health Planning: Supports genetic surveillance to inform public-health and safety decisions and targeted interventions during outbreaks.

Methodology:

Processes nanopore long-read sequencing data (NST), addresses nanopore accuracy challenges via analytical techniques, and integrates phylogenetic-tree and hotspot mutation analyses.