Nanometa Live

Nanometa Live performs real-time metagenomic data analysis and pathogen identification utilizing Oxford Nanopore Technologies' MinION and Flongle flow cells.

Key Features:

• Automated BLAST validation: The tool includes automated BLAST validation for taxonomic assignments.
• Custom Kraken2 database handling: The tool provides streamlined handling of custom Kraken2 databases.
• Offline operation: The tool runs without constant internet or server access once installed.
• Taxonomic composition visualization: The tool provides a comprehensive view of taxonomic composition and facilitates detection of user-defined pathogens or other species of interest.

Scientific Applications:

• Real-time metagenomic analysis: The tool enables real-time analysis and visualization of metagenomic data as it is being generated.
• Pathogen identification: The tool facilitates detection of user-defined pathogens or other species of interest from nanopore sequencing data.

Methodology:

The tool is implemented as a local web application using the Dash framework with Snakemake handling data processing.