NanoPASS

NanoPASS simulates nanoparticle diffusion and sedimentation in cell culture wells to estimate the time-resolved effective nanoparticle dose contacting cells for in vitro dosimetry and nanotoxicology.

Key Features:

• Simulation of sedimentation: Models three-dimensional diffusion and sedimentation of nanoparticles within cell culture wells.
• Dose estimation: Calculates the fraction and time-resolved concentration of nanoparticles that contact cells to estimate effective in vitro doses.
• Physicochemical integration: Incorporates nanoparticle physicochemical properties and cell culture system parameters to modulate diffusion and sedimentation behavior.

Scientific Applications:

• Nanotoxicology: Provides dosimetric calculations to improve experimental comparability and interpretation of in vitro nanoparticle toxicity and safety assessments.

Methodology:

Implements the 3DSDD (Three-Dimensional Sedimentation Dosimetry) model to account for diffusion and sedimentation characteristics of nanoparticles as influenced by their physicochemical properties and the cell culture system.