NanoPlot

NanoPlot visualizes and summarizes long-read sequencing data to assess read length, quality, and distribution for quality control and downstream analysis.

Key Features:

• Data Compatibility: Accepts BAM, CRAM, FASTQ, FASTA, and platform-specific TSV summary formats for input.
• Visualization Capabilities: Generates a range of plots tailored to long-read sequencing data that reveal read length, quality, and distribution characteristics.

Scientific Applications:

• Long-read sequencing quality control: Processes and visualizes data from Oxford Nanopore Technologies and Pacific Biosciences to evaluate read length and base-call quality distributions.
• Sequencing run optimization: Provides visual summaries of read quality and length distributions to inform adjustments to sequencing protocols and run conditions.
• Interpretation of complex genomic regions: Uses read-level visualizations to aid examination of data characteristics relevant to analyzing complex genomic loci.

Methodology:

Implemented in Python3.