Nanopore

Nanopore analyzes Oxford Nanopore sequencing data to directly read native DNA and RNA molecules for long-read genomic, transcriptomic, and epigenomic analyses.

Key Features:

• Direct sequencing of native molecules: Sequences native DNA and RNA, including mRNA, without conversion to cDNA to preserve full-length molecules.
• Long-read sequencing: Produces extremely long reads suitable for resolving full-length transcripts, complex genomic regions, structural variants, and repetitive sequences.
• Epigenetic modification detection: Detects and documents DNA and RNA base modifications for epigenomic studies.
• Amplification-free workflow: Bypasses nucleic acid amplification or synthesis during sequencing to retain native molecule information.
• Data analysis and error mitigation: Implements computational approaches to address nanopore data analysis complexity and to mitigate error rates, enhancing accuracy and reliability.

Scientific Applications:

• Genomic research: Characterizes complex genomic regions, structural variants, and repetitive sequences using long reads.
• Transcriptomic studies: Analyzes full-length RNA transcripts to study gene expression patterns and alternative splicing.
• Epigenomics: Profiles DNA and RNA base modifications that influence gene regulation and cellular processes.

Methodology:

Uses the Oxford Nanopore direct sequencing approach to read native DNA and RNA without amplification or synthesis and applies computational methods to handle nanopore data complexity and reduce error rates.