NanoRtax

NanoRTax is a taxonomic and diversity analysis pipeline built originally for Nanopore 16S rRNA data with real-time analysis support in mind. It combines state-of-the-art classifiers such as Kraken2, Centrifuge and BLAST with downstream analysis steps to provide a framework for the analysis of in-progress sequencing runs. NanoRTax retrieves the final output files in the same structure/format for every classifier which enables more comprehensive tool/database comparison and better benchmarking capabilities. Additionally, NanoRTax includes a web application (./viz_webapp/) for visualizing complete or partial pipeline outputs. The NanoRTax pipeline is built using Nextflow, a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with conda environments and docker containers making installation trivial and results highly reproducible.