NaViA

NaViA analyzes complex native mass spectra to assist assignment and interpretation of protein complex composition, subunit stoichiometry, and ligand binding.

Key Features:

• Raw data processing: Processes raw native mass spectrometry spectral data for downstream analysis.
• Peak assignment augmentation: Augments manual peak assignment by supporting peak detection and annotation.
• Mass–stoichiometry matching: Matches measured masses to the stoichiometry of component subunits within protein complexes.
• Annotation and sharing: Generates spectrum annotations and supports sharing mass spectra together with their interpretations.

Scientific Applications:

• Protein complex composition analysis: Determines protein complex composition and subunit arrangements from native MS data.
• Subunit stoichiometry determination: Infers subunit stoichiometry by matching observed masses to subunit combinations.
• Ligand binding characterization: Detects mass shifts in native MS spectra to characterize ligand binding to protein complexes.

Methodology:

Performs raw data processing, peak assignment, annotation, mass-to-stoichiometry matching, and supports sharing of spectra with their interpretations.