nf-core isoseq

nf-core isoseq processes and annotates PacBio Iso-Seq RNA long-read sequencing data to identify and characterize full-length transcripts and isoforms.

Key Features:

• Automatic Read Processing: Automates processing of raw Iso-Seq reads through a series of pipeline steps to prepare data for annotation.
• Genome Annotation: Annotates full-length transcripts and isoforms derived from PacBio Iso-Seq data.
• Reproducibility and Portability: Implements the nf-core framework and guidelines with minimal dependencies to enable reproducible execution across computational platforms.

Scientific Applications:

• Transcript and Isoform Identification: Identification and characterization of full-length transcripts and alternative isoforms from Iso-Seq long-read data.
• Reference-guided Transcriptome Annotation: Annotation of transcriptomes from long-read sequencing while reducing reliance on short-read assembly workflows.

Methodology:

Automated read processing and genome annotation implemented within the nf-core framework following nf-core guidelines for minimal dependencies and reproducibility.