NIBR 2D-PAGE

NIBR 2D-PAGE integrates gel-based proteomics (two-dimensional electrophoresis, 2-D PAGE) datasets across the World-2DPAGE Constellation hosted on the ExPASy server to support protein identification and proteome mapping.

Key Features:

• World-2DPAGE List: Aggregates a comprehensive list of existing 2-D PAGE database servers worldwide.
• World-2DPAGE Portal: Enables simultaneous queries across multiple proteomics databases within the World-2DPAGE Constellation.
• World-2DPAGE Repository: Stores gel-based proteomics data linked to protein identifications published in the literature and is implemented using the Make2D-DB package to produce SWISS-2DPAGE-like databases.

Scientific Applications:

• Protein identification: Facilitates mapping of 2-D PAGE spots to protein identifications reported in the literature and databases.
• Comparative proteomics: Supports cross-database comparisons of gel-based proteome maps across experiments and servers.
• Biomarker discovery: Enables aggregation and comparison of gel-resolved protein data to assist in candidate biomarker detection.
• Meta-analysis: Permits integration of distributed 2-D PAGE datasets for aggregated analyses across studies.

Methodology:

Implements the Make2D-DB package to construct SWISS-2DPAGE-like repositories and supports simultaneous querying and aggregation across distributed 2-D PAGE servers within the World-2DPAGE Constellation hosted on the ExPASy server.