Non-B DB

Non-B DB catalogs genome-wide predictions of non-canonical (non-B) DNA structures to support research into their genomic locations and potential roles in regulation, evolution, and disease.

Key Features:

• Motif coverage: Genome-wide predictions include Z-DNA motifs, quadruplex-forming sequences, inverted repeats, mirror repeats, direct repeats, cruciforms, triplexes, and slipped structures.
• Disease-associated motifs: Annotations identify motifs predicted to form static DNA bends, short tandem repeats, and homo(purine•pyrimidine) tracts that have been linked to disease-related loci.
• Genomic integration and species coverage: Predictions and annotations are mapped to the latest releases of human, chimp, dog, macaque, and mouse genomes and integrated with genes, single-nucleotide polymorphisms (SNPs), SINEs, and LINEs.

Scientific Applications:

• Gene regulation studies: Locate non-B DNA motifs to investigate their potential effects on gene expression and regulatory element function.
• Chromatin and genome stability research: Assess associations between non-canonical DNA structures and chromatin organization or genome instability.
• Disease mechanism investigation: Prioritize motifs and loci for studying molecular mechanisms underlying genetic disorders linked to non-B DNA structures.
• Comparative genomics: Compare motif distributions across human, chimp, dog, macaque, and mouse genomes to study evolutionary conservation and divergence.

Methodology:

Genome-wide predictions of non-B DNA motifs were generated and mapped to the latest releases of human, chimp, dog, macaque, and mouse genomes and annotated with genes, single-nucleotide polymorphisms (SNPs), SINEs, and LINEs.