NovoLign

NovoLign performs sequence alignment of de novo sequences to enable rapid taxonomic profiling and evaluation of database searching outcomes in metaproteomics.

Key Features:

• De novo sequence alignment: Performs large-scale sequence alignment of de novo sequences from complete metaproteomics experiments.
• Rapid taxonomic profiling: Enables rapid taxonomic profiling of complex microbial communities without requiring sample-specific databases from whole metagenome sequencing.
• Database search evaluation: Evaluates the taxonomic coverage of metaproteomics outcomes obtained from database searches.
• Reference database creation: Supports the creation of reference sequence databases for database searching to ensure comprehensive coverage.

Scientific Applications:

• Metaproteomics of complex communities: Provides rapid and orthogonal insights into metaproteomics data from complex microbial communities including environmental microbial communities, synthetic communities, laboratory enrichment cultures, and pure reference strains.
• Taxonomic coverage assessment: Assesses taxonomic coverage and false positive annotations across diverse in silico and experimental datasets.

Methodology:

NovoLign employs large-scale sequence alignment of de novo sequences from complete metaproteomics experiments.