NT-seq

NT-seq maps DNA methylation across prokaryotic genomes, enabling simultaneous profiling of N^6-methyladenine (6mA), N^4-methylcytosine (4mC), and 5-methylcytosine (5mC) for comprehensive methylome analysis.

Key Features:

• Simultaneous Mapping: Maps N^6-methyladenine (6mA), N^4-methylcytosine (4mC), and 5-methylcytosine (5mC) in prokaryotic genomes within a single workflow.
• Reliability: Detects all known methylation motifs in bacterial genomes.
• De Novo Motif Identification: Identifies novel methylation motifs de novo from sequence data.
• Chemical-based Sequencing: Employs a chemical-based sequencing approach to enable detection of 6mA, 4mC, and 5mC.

Scientific Applications:

• Genomic Studies: Characterizing the distribution and motifs of 6mA, 4mC, and 5mC across bacterial genomes.
• Epigenetic Research: Investigating how prokaryotic DNA methylation influences gene expression, genome stability, and cellular processes.

Methodology:

NT-seq employs a chemical-based sequencing approach to map N^6-methyladenine (6mA), N^4-methylcytosine (4mC), and 5-methylcytosine (5mC).