NTMG

NTMG generates multiplex PCR primer sets from cDNA to produce N-terminal truncated mutants for mapping protein functional domains.

Key Features:

• Automated primer generation: Automatically generates groups of forward and reverse PCR primers tailored for multiplex PCR experiments.
• ATG-like codon identification: Computationally identifies ATG-like codons within cDNA sequences to define truncation start sites.
• Multiplex PCR primer design: Designs primer sets that enable simultaneous production of multiple N-terminal truncated variants in a single multiplex PCR.
• Support for sequential deletion mapping: Produces defined N-terminal truncations to facilitate sequential deletion approaches for mapping protein functional domains.

Scientific Applications:

• Functional domain analysis: Facilitates sequential deletion mapping of protein functional domains via generated N-terminal truncations.
• Gene expression regulation studies: Enables investigation of protein roles relevant to gene expression regulation using truncation variants.
• Protein engineering and structural biology: Provides defined truncation variants useful for protein engineering and structural biology experiments.

Methodology:

Computational identification of ATG-like codons within input cDNA sequences and design of forward and reverse primers based on those codons for multiplex PCR.