nucloc

nucloc generates high-resolution probabilistic maps of genomic loci positions in budding yeast nuclei to analyze subnuclear gene territories and their remodeling during transcriptional activation.

Key Features:

• High-Resolution Probabilistic Mapping: Generates probabilistic maps that detail subnuclear domains occupied by individual genomic loci across thousands of living budding yeast cells.
• Automated Image Processing: Performs automated analysis of fluorescence microscopy images to produce the probabilistic maps and enable large-scale sampling.
• Gene Territory Identification: Identifies gene territories that are significantly smaller than the nucleus and detects their remodeling in response to transcriptional activation.
• Nucleolus as a Landmark: Uses the nucleolus as a critical reference landmark for positioning genomic loci within the nucleus.

Scientific Applications:

• Visualization and Quantification: Enables visualization and quantitative analysis of gene territory positions relative to each other and to nuclear landmarks.
• Functional Genomics Studies: Applied to specific genes, including those involved in galactose metabolism and ribosome biogenesis, to study the relationship between gene positioning and cellular processes.

Methodology:

Creates detailed probabilistic maps through automated processing of fluorescence microscopy images and comprehensive sampling of nuclear organization across large numbers of cells.