O-GlcNAcAtlas

O-GlcNAcAtlas provides a curated database of experimentally identified O-GlcNAc sites and proteins to support site-specific functional studies and computational analyses of protein O-GlcNAcylation.

Key Features:

• Coverage timeframe: Integrates experimentally identified O-GlcNAc sites and associated proteins reported from 1984 to December 2019.
• Datasets: Separates entries into Dataset-I (unambiguously identified O-GlcNAc sites) and Dataset-II (ambiguously identified sites).
• Protein count: Compiles a total of 4571 O-GlcNAc-modified proteins across the datasets.
• Modification specificity: Records O-GlcNAcylation occurring on serine/threonine residues.
• Annotation fields: Provides gene name, organism, modification sites, site mapping methods, and literature references for each protein entry.
• Sequence mapping: Maps reported O-GlcNAc peptide sequence identity to UniProtKB protein entries to address data heterogeneity.
• Manual curation: Assembles experimentally validated site information through curated literature compilation.

Scientific Applications:

• Site-specific functional studies: Enables experimental investigation of specific O-GlcNAc modification sites on proteins.
• Computational analyses: Supports computational studies related to protein O-GlcNAcylation, including site-centric analyses.
• Disease research: Facilitates study of O-GlcNAcylation implications in cancer, diabetes, and neurodegenerative disorders.

Methodology:

Collected experimentally identified O-GlcNAc sites and associated proteins from published literature (1984–December 2019), classified sites into Dataset-I (unambiguous) and Dataset-II (ambiguous), and mapped reported O-GlcNAc peptide sequences to UniProtKB protein entries by sequence identity.