OAS

OAS provides cleaned, annotated, and translated unpaired and paired antibody sequences derived from raw FASTQ B-cell receptor (BCR) repertoire sequencing to support analysis of nucleotide and amino-acid antibody repertoires, including SARS-CoV-2 datasets.

Key Features:

• Data cleaning and annotation: Cleans raw FASTQ BCR sequencing reads and supplies nucleotide and amino-acid sequences annotated with metadata and flags for potential issues.
• MIAIRR compliance: Annotations adhere to the Minimal Information about Adaptive Immune Receptor Repertoire (MIAIRR) standards.
• Paired VH/VL sequences: Includes paired variable heavy (VH) and light (VL) chain sequence data alongside unpaired sequences.
• Comprehensive study coverage: Expands dataset coverage with 25 additional studies, including datasets related to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

Scientific Applications:

• Disease mechanism analysis: Enables investigation of antibody repertoire changes associated with different disease states.
• Vaccine response characterization: Supports molecular-level analysis of immune responses elicited by vaccination.
• Therapeutic antibody discovery: Provides natural repertoire sequences for exploration in antibody-based therapeutic development.

Methodology:

Processes raw FASTQ B-cell receptor repertoire sequencing data by cleaning reads, translating nucleotide sequences to amino acids, and annotating sequences with MIAIRR-compliant metadata and flags for potential issues.