OBITools

OBITools provides a suite of Python programs for manipulating and analyzing next-generation sequencing (NGS) DNA metabarcoding sequence files from platforms such as 454 and Illumina.

Key Features:

• Python-based programs: A collection of Python programs for manipulation and analysis of sequence files.
• Sequence Data Management: Tools for filtering and editing sequences to prepare large NGS datasets for downstream analysis.
• Taxonomic Annotation Integration: Incorporates taxonomic annotation into the processing pipeline to preserve biological context for species identification and biodiversity assessment.
• Customizable Analysis Pipelines: Supports creation of tailor-made analysis pipelines adaptable to specific research questions and experimental designs.

Scientific Applications:

• Biodiversity Surveys: Analysis of complex ecological communities and identification of species presence and abundance from environmental DNA samples.
• Diet Analyses: Examination of dietary habits by analyzing gut content or fecal samples for prey DNA.

Methodology:

Sequence file manipulation including sequence filtering and editing, integration of taxonomic annotation, and construction of customizable analysis pipelines for NGS data (454 and Illumina).