Oli2go

Oli2go designs primers and hybridization probes for multiplex applications and evaluates specificity and cross-dimer interactions to produce target-specific oligonucleotides.

Key Features:

• Multiplex design capability: Designs primers and hybridization probes for multiplex applications, enabling simultaneous use of multiple oligonucleotides.
• Comprehensive specificity checks: Performs specificity assessments against bacteria, viruses, fungi, invertebrates, plants, protozoa, archaea, whole genome shotgun projects, and environmental samples to minimize off-target binding.
• Advanced cross-dimer analysis: Conducts cross-dimer checks on individual primers and in an all-against-all manner to prevent unintended interactions between oligonucleotides.
• Automated workflow: Automates the oligonucleotide design workflow and integrates parameter-driven primer and probe generation.

Scientific Applications:

• Multiplex amplification and detection: Supports experiments requiring simultaneous amplification or detection of multiple targets.
• qPCR and quantitative assays: Enables qPCR assay design with specificity and cross-dimer checks to improve accuracy in quantitative measurements.
• Microarray and high-throughput genomics: Facilitates probe design and validation for microarray analyses and other high-throughput genomic applications.

Methodology:

Automated primer and probe design based on user-defined parameters; specificity checks against diverse genetic databases including bacteria, viruses, fungi, invertebrates, plants, protozoa, archaea, whole genome shotgun projects, and environmental samples; individual and all-against-all cross-dimer analysis.