omiRas

omiRas analyzes non-coding RNAs from small RNA-sequencing experiments to annotate, compare, and visualize ncRNA interaction networks across two conditions.

Key Features:

• Data Submission and Processing: Processes raw small RNA-seq data to generate comprehensive analysis outputs.
• Static Annotation Results: Provides static annotation outputs including length distribution of ncRNAs, mapping statistics to known RNA databases, alignment files and quantification tables per library, and lists of ncRNAs differentially expressed between the two conditions.
• Interactive Network Visualization: Generates network visualizations of selected microRNAs (miRNAs) and their target genes by integrating multiple miRNA–mRNA interaction databases.

Scientific Applications:

• ncRNAome profiling: Profiles ncRNA classes including transfer RNA, small nucleolar RNA, and microRNA (miRNA) from small RNA-seq data.
• Differential expression analysis: Identifies ncRNAs differentially expressed between two conditions to highlight condition-specific molecular changes.
• Regulatory network analysis: Constructs miRNA–mRNA interaction networks by integrating multiple miRNA–mRNA interaction databases to explore regulatory relationships.

Methodology:

Processes raw small RNA-seq data, computes ncRNA length distributions, maps sequences against known RNA databases, produces alignment files and quantification tables per library, identifies ncRNAs differentially expressed between two conditions, and integrates multiple miRNA–mRNA interaction databases to build network visualizations.