OneFlowTraX

OneFlowTraX automates single-molecule localization, tracking, and clustering analysis for super-resolution microscopy data including single-particle tracking photoactivated localization microscopy (sptPALM).

Key Features:

• Single-molecule localization: Performs automated localization of individual molecules from super-resolution microscopy images.
• Single-particle tracking: Tracks localized molecules over time to analyze their dynamics.
• Mobility analysis: Extracts diffusion parameters from tracked single molecules.
• Clustering analysis: Identifies and quantifies nanoscale molecular clusters.
• High-throughput processing: Analyzes millions of molecules in a few minutes.

Scientific Applications:

• Plant plasma membrane protein dynamics: Analyzes diffusion and clustering of proteins such as aquaporins, the brassinosteroid receptor BRI1 (Brassinosteroid Insensitive 1), and RLP44 (Receptor-Like Protein 44).
• Multi-fluorophore compatibility: Supports analysis with mEos3.2, PA-GFP, and PATagRFP fluorophores.
• Live cell nanoscale organization: Studies macromolecular assembly organization and single-molecule dynamics in living cells.

Methodology:

The software implements automated algorithms for single-molecule localization, tracking, mobility analysis, and clustering analysis specifically optimized for single-molecule localization microscopy approaches.