OralImmunoAnalyser

OralImmunoAnalyser quantifies immunohistochemical staining in oral leukoplakia by automatically counting cells across three staining levels and epithelial layers to estimate ki67 expression.

Key Features:

• Automatic cell detection: Segments and classifies cells in immunohistochemical images with 64.4% sensitivity and 93% specificity for automatic detection.
• Three-level staining classification: Counts cells in three staining intensity levels (negative, weak, strong).
• Epithelial layer stratification: Quantifies cells separately within each epithelial layer.
• Expert verification workflow: Allows investigators to oversee and correct automatically recognized cells before final quantification.

Scientific Applications:

• Oral leukoplakia assessment: Supports diagnosis and prognosis of potentially malignant oral disorders by quantifying ki67 as a cell proliferation biomarker.
• Dysplasia evaluation: Complements histopathological examination by providing reproducible cell proliferation measurements to aid in epithelial dysplasia grading.

Methodology:

Combines automatic image processing with segmentation and classification models; cell classification achieves 79.8% accuracy.