P-SAMS

P-SAMS automates the design of artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for targeted gene silencing in plant systems.

Key Features:

• P-SAMS amiRNA Designer: Automated design of artificial microRNAs (amiRNAs) for targeting plant genes.
• P-SAMS syn-tasiRNA Designer: Automated design of synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for multiplexed or specific gene targeting.
• Automated small RNA design: Computational generation of candidate small RNA sequences for targeted gene silencing.
• Cloning oligonucleotides output: Production of the sequences of two oligonucleotides required for cloning into 'B/c' compatible vectors.
• Plant-targeted application: Designs explicitly intended for use in plant systems and plant gene regulation studies.

Scientific Applications:

• Targeted gene silencing: Generation of amiRNAs and syn-tasiRNAs for specific knockdown of plant genes.
• Functional genomics: Use in studies to probe gene function and regulatory networks in plants.
• Molecular breeding: Application to modify gene expression for crop trait improvement.
• Plant biotechnology: Support for biotechnological approaches that require designed small RNAs in botanical research.

Methodology:

Automated generation of amiRNA and syn-tasiRNA sequences and the corresponding two oligonucleotides for cloning into 'B/c' compatible vectors.