PanCircBase

PanCircBase catalogs circular RNAs (circRNAs) expressed in pancreatic islets and provides annotations and experimental reagents to support studies of circRNA roles in β-cell gene regulation and insulin biosynthesis and secretion.

Key Features:

• Pancreatic Islet CircRNA Annotation: Detailed annotations per circRNA including genomic position, host gene, exon details, splice length, sequence data, other database IDs, and cross-species conservation.
• Divergent Primers for PCR Analysis: Precomputed divergent primer sequences for polymerase chain reaction validation of circRNAs.
• siRNAs for Target Silencing: Small interfering RNA sequences provided for targeted silencing of circRNAs.
• miRNA Associations: Associations between circRNAs and microRNAs (miRNAs) indicating potential regulatory interactions.
• Protein-Coding CircRNAs: Identification of putative protein-coding circRNAs and their corresponding polypeptides.

Scientific Applications:

• β-cell regulatory mechanism studies: Supports investigation of circRNA-mediated regulation of gene expression in pancreatic β-cells.
• Insulin biosynthesis and secretion research: Enables studies on circRNA involvement in insulin production and secretion pathways.
• Biomarker and therapeutic target discovery: Facilitates identification of circRNAs as potential biomarkers or therapeutic targets in pancreatic disease and diabetes research.

Methodology:

The database was constructed by analyzing publicly available RNA-sequencing datasets from pancreatic islets to identify and catalog circRNAs.