PeakTrace

PeakTrace improves basecalling accuracy and extends read length for Sanger DNA sequencing trace files, producing higher-quality nucleotide sequences from ABI DNA sequencers (310, 3700, 3100, 3130, 3730, 3500) and MegBACE platforms.

Key Features:

• Improved quality and read length: Produces higher-quality nucleotide sequences and extended read lengths from Sanger trace files.
• Sequencer compatibility: Processes trace files from ABI DNA sequencers 310, 3700, 3100, 3130, 3730, and 3500, and from MegBACE sequencers.
• Basecalling algorithm: Applies advanced algorithms to convert raw electropherogram trace files into nucleotide sequences.
• Quality control: Includes built-in quality control mechanisms to ensure the reliability of basecalled sequences.

Scientific Applications:

• Genotyping and pathogen identification: Supports multilocus sequence typing (MLST) and identification of pathogens such as Salmonella enterica serovar Typhimurium sequence type (ST) 313 associated with invasive non-typhoidal salmonella (iNTS) disease in sub-Saharan Africa.
• Epidemiological studies: Facilitates sequencing and genotyping for studies of pathogen distribution and prevalence in populations.

Methodology:

Employs advanced algorithms to interpret raw sequencing trace files and convert them into nucleotide sequences, and implements built-in quality control mechanisms to assess and ensure the reliability of basecalled sequences.