Prime Editing Guide Generator (PEGG)

Prime Editing Guide Generator (PEGG) generates prime editing guide RNAs (pegRNAs) and pegRNA-sensor libraries for high-throughput prime editing screens.

Key Features:

• High-throughput pegRNA design: Enables rapid generation of custom pegRNA libraries for pooled screens.
• PegRNA-sensor library generation: Produces pegRNA-sensor libraries for calibration of guide editing activity.
• Python package implementation: Provides a fast, flexible computational framework for automated pegRNA design.

Scientific Applications:

• Prime editing screens: Supports high-throughput prime editing screens through generation of custom pegRNA-sensor libraries.
• Guide editing activity calibration: Facilitates calibration of guide editing activity in pooled screens using pegRNA-sensor libraries.

Methodology:

PEGG is implemented as a Python package for automated computational design of pegRNAs and pegRNA-sensor libraries.