peplife

peplife provides a curated database of experimentally determined peptide half-lives to support analysis of peptide stability and effects of chemical modifications.

Key Features:

• Extensive Data Collection: 2,229 entries covering 1,193 unique therapeutic peptides, with each entry listing peptide name, sequence, experimentally determined half-life, reported modifications, and the assay used for half-life determination.
• Comprehensive Molecular and Biological Annotations: Entries include biological nature and activity annotations, SMILES notation, and structural representations.
• Integrated Analytical Tools: Integration of bioinformatics tools including BLAST, the Smith-Waterman algorithm, GGSEARCH, Jalview, and MUSTANG for sequence alignment and structural analysis.

Scientific Applications:

• Modification Effects on Stability: Analysis of how chemical modifications, D-amino acid substitutions, cyclization, and replacement of labile amino acids influence peptide half-life.
• Factors Affecting Therapeutic Efficacy: Investigation of impacts of sequence length, delivery routes, and other variables on peptide stability and therapeutic performance.

Methodology:

Curation of experimental half-life data and integration of analytical tools (BLAST, Smith-Waterman, GGSEARCH, Jalview, MUSTANG), with inclusion of SMILES notation and structural representations.