PepPSy

PepPSy prioritizes predicted human protein-coding genes using gene expression and other 'omics' data to identify human tissues likely to express previously unseen proteins, supporting validation of approximately 3,000 of 20,000 predicted human gene products at the protein level.

Key Features:

• Gene expression-based prioritization: Ranks genes by their expression profiles across various human tissues to indicate likelihood of protein presence.
• 'Omics data integration: Leverages existing 'omics datasets to inform prioritization and tissue-specific expression inferences.
• Reannotation support: Assists biocurators in revisiting and refining annotations for specific categories of proteins using expression evidence.

Scientific Applications:

• Protein Validation: Prioritizes dubious or unvalidated protein-coding genes to guide experimental confirmation at the protein level.
• Tissue-Specific Research: Identifies which human tissues are most likely to express particular proteins to optimize experimental design and resource allocation.
• Database Integration (neXtProt): Provides expression-based evidence to support reannotation and integration with databases such as neXtProt.

Methodology:

PepPSy uses a database of gene expression and other 'omics' data to rank protein-coding genes by their likelihood of expression in specific human tissues.