PerturbAtlas

PerturbAtlas re-analyzes publicly archived RNA-seq libraries to provide quantitative data on gene expression, transcript profiles, and alternative splicing events following genetic perturbation.

Key Features:

• Large-scale curation: Re-analyzes a curated set of 122,801 RNA-seq libraries across 13 species.
• Multi-level analysis: Provides data on gene expression, transcript profiles, and alternative splicing events following genetic perturbation.
• Forward and reverse search approaches: Enables exploration of perturbation consequences and identification of potential causal perturbations.
• Search functionality: Allows searches by species, tissue, or genomic features.

Scientific Applications:

• Gene function studies: Assists in identifying trends at the gene and isoform levels in perturbation assays to understand gene function.
• Perturbation consequence analysis: Provides detailed information on alterations following perturbations for exploring downstream mechanisms.
• Causal perturbation identification: Enables identification of potential causal perturbations through reverse approaches.

Methodology:

PerturbAtlas re-analyzes a curated set of publicly archived RNA-seq libraries with standardized metadata formatting.