Pharokka

Pharokka annotates bacteriophage genomes and metagenomes, producing standardized genome annotations to support analyses in phage biology, ecology, and comparative genomics.

Key Features:

• Phage-specific annotation: Targets bacteriophage genomes to provide annotations tailored to phage genetic content.
• Rapid processing: Processes an average 50-kb bacteriophage genome in under five minutes.
• Standards compliance: Generates outputs that adhere to established annotation standards for compatibility in downstream analyses.

Scientific Applications:

• Genome and metagenome annotation: Produces standardized annotations for individual phage genomes and phage sequences within metagenomes.
• Phage biology, ecology, and evolution: Supports studies of gene content, functional repertoire, and evolutionary relationships among bacteriophages.
• High-throughput comparative analyses: Enables consistent annotations across multiple phage genomes for comparative genomics and large-scale phage surveys.

Methodology:

Implemented in Python.