Phospho-Analyst

Phospho-Analyst performs statistical analysis and normalization of phosphoproteomics data to identify differential phosphorylation events and relate them to underlying protein abundance.

Key Features:

• Integration with MaxQuant: Integrates with data processed by MaxQuant to accept quantitative phosphopeptide and protein outputs.
• Normalization to total protein abundance: Performs automatic normalization of phosphosite intensities to underlying protein abundance when quantitative total proteomic data for the same samples are provided.
• Differential expression analysis: Performs differential expression analyses to identify significant changes in phosphorylation events.
• Visualization tools: Provides visualization capabilities for interpreting phosphoproteomics results.

Scientific Applications:

• Phosphopeptide and protein quantification: Enables comprehensive identification and quantification of phosphorylated peptides and proteins.
• Signal transduction analysis: Supports exploration of changes in signal transduction pathways and cellular signaling processes via phosphorylation changes.
• Disease mechanism and target discovery: Supports investigation of disease mechanisms, drug target identification, and biomarker discovery through differential phosphorylation analysis.

Methodology:

Integration of MaxQuant-processed datasets, normalization against total protein abundance when available, and differential expression analyses to identify significant phosphorylation changes.