Phosprof

Phosprof analyzes drug-induced protein phosphorylation profiles from MCF7 and K562 cell lines treated with 94 clinically relevant drugs to characterize pathway activities and kinase regulatory mechanisms.

Key Features:

• Phosphorylation Profile Analysis: Quantifies upregulation and downregulation of phosphorylated signature proteins across MCF7 and K562 cell lines treated with 94 clinically relevant drugs.
• Pathway Visualization: Presents significant signal transduction pathways in a hierarchical tree format to show how specific drugs perturb pathway components.
• Drug Response Database: Catalogs drug responses based on phosphorylation activities and enables comparison of molecular similarities among the 94 included drugs.

Scientific Applications:

• Cancer drug response analysis: Investigates how clinical drugs alter phosphorylation pathways in cancer-relevant cell lines to inform mechanism-of-action studies.
• Kinase regulatory mechanism characterization: Supports analysis of kinase-targeted perturbations to elucidate regulatory mechanisms and inform therapeutic targeting.

Methodology:

Phosphorylation profiles were measured using a previously established experimental platform on MCF7 and K562 cell lines treated with a panel of 94 clinical drugs.