PhylOligo

PhylOligo identifies contaminant and untargeted organism sequences in genome assemblies by analyzing DNA compositional characteristics to separate and extract organism-specific sequences.

Key Features:

• DNA compositional analysis: Analyzes DNA compositional characteristics to distinguish sequences originating from different organisms.
• Contaminant detection and segregation: Identifies and segregates contaminant and untargeted organism sequences within genome assemblies.
• Sequence extraction: Extracts organism-specific sequences from mixed-genome assemblies.
• Applicability to complex samples: Applicable to complex and non-model organism sequencing projects, including co-assembled species.
• Implementation: Implemented using Python 3 and R.

Scientific Applications:

• Contaminant screening: Detects contaminant and untargeted organism sequences in genome assemblies.
• Target recovery from mixed assemblies: Enables extraction of target organism sequences from mixed or co-assembled genomes.
• Assembly quality assurance: Supports assessment and improvement of genome assembly accuracy and integrity in complex sequencing projects.

Methodology:

Computational analysis of DNA compositional characteristics to identify sequences belonging to different organisms.