PHYSICO

PHYSICO computes physicochemical, window-dependent, and homologous position-based substitution (PWS) properties from protein sequences to characterize sequence variability, diversity, and evolutionary conservation for studies in molecular evolution, functional genomics, and comparative proteomics.

Key Features:

• Comprehensive property extraction: Computes physicochemical characteristics, window-dependent attributes, and homologous position-based substitution (PWS) features from protein sequences.
• Positional and BLOCK-specific analysis: Assesses positional diversity and BLOCK-specific diversity and conservation metrics.
• Automated Block Preparation: Generates BLOCK-FASTA files via an Automated Block Preparation tool.
• Output formats: Exports analysis results in an itemized Excel format.

Scientific Applications:

• PWS analysis: Performs homologous position-based substitution analysis of protein sequences.
• Molecular evolution: Supports investigations of evolutionary properties and constraints in protein families.
• Functional genomics: Relates physicochemical and substitution-based properties to protein function in genomic contexts.
• Comparative proteomics: Enables comparative analyses of protein properties across proteomes.
• Protein function and stability studies: Facilitates exploration of protein function and stability using computed physicochemical and substitution features.

Methodology:

Computes physicochemical and window-dependent properties, extracts homologous position-based substitution (PWS) features, assesses positional and BLOCK-specific diversity and conservation, generates BLOCK-FASTA files with an Automated Block Preparation tool, and exports results in itemized Excel format.