PhysioFit

PhysioFit calculates extracellular fluxes and growth parameters in metabolic systems using any growth model.

Key Features:

• Flexible growth modeling: Includes common growth models and allows advanced users to implement additional models for alternative kinetics.
• Multiple interfaces: Functions as a Python library, command-line interface, and graphical user interface.
• Interoperable design: Built for integration into existing computational pipelines.

Scientific Applications:

• Systems biology: Quantifies growth parameters and extracellular uptake and production fluxes from time-course measurements.
• Synthetic biology: Supports metabolic systems analysis with customizable growth models for diverse experimental setups.

Methodology:

Fluxes are estimated by fitting mathematical models to time-course measurements of cell concentration and extracellular substrate and product concentrations.