PICKLES

PICKLES aggregates and enables analysis of genome-scale CRISPR knockout fitness screens to characterize gene essentiality across human cell lines and tissue types.

Key Features:

• Integrated CRISPR libraries: Integrates data from the Avana, Score, and TKOv3 whole-genome CRISPR libraries.
• Data coverage: Compiles results from 1,162 whole-genome screens covering knockout fitness phenotypes for 18,959 genes.
• Gene-level fitness profiles: Provides gene knockout fitness phenotypes across diverse human cell lines and tissue types.
• Comparative analysis: Enables comparison of knockout fitness profiles with gene expression and mutation profiles.

Scientific Applications:

• Gene Function Analysis: Identification and characterization of essential genes from knockout fitness phenotypes.
• Comparative Genomics: Integration of fitness profiles with expression and mutation data to study gene interactions and regulatory mechanisms.
• Drug Target Identification: Prioritization of genes critical for cell survival as potential therapeutic targets.

Methodology:

Leverages pooled genome-scale CRISPR knockout libraries (Avana, Score, TKOv3) to systematically disrupt gene function and compiles screen results into a centralized database for high-throughput analysis.