Picopore

Picopore compresses and manages large datasets generated by Oxford Nanopore Technologies' MinION and PromethION long-read sequencing platforms to reduce storage requirements while preserving biologically relevant information.

Key Features:

• Lossless compression: Performs lossless compression with an average dataset size reduction of 25% while maintaining complete data fidelity.
• Deep lossless compression: Provides a deep lossless option with an average size reduction of 44% while preserving all original data.
• Raw compression: Offers a raw method that prioritizes retention of biologically relevant information with an average size reduction of 88%.
• Real-time operation: Operates concurrently with sequencing runs to reduce immediate storage demands during MinION and PromethION experiments.
• Redundant file format targeting: Targets highly redundant file formats produced by ONT long-read sequencing to reduce both live-run and archival storage footprints.

Scientific Applications:

• On-run storage reduction: Reduces real-time storage demands on MinION-capable laptops and sequencing systems during data acquisition.
• Archival storage reduction: Lowers long-term archival storage requirements for datasets generated by MinION and PromethION.
• Large-scale dataset management: Enables more storage-efficient handling of large-scale long-read sequencing datasets for genomic research.

Methodology:

Implements three explicitly stated compression methodologies—lossless, deep lossless, and raw—and supports real-time compression concurrent with sequencing runs.