PIMBA

PIMBA performs DNA metabarcoding analyses of environmental samples to assign taxonomy and quantify biodiversity from Next-Generation Sequencing (NGS) data.

Key Features:

• Customizable Database Integration: PIMBA allows incorporation of custom databases alongside established reference databases to increase taxonomic coverage and detect specific or rare taxa.
• Comprehensive Analysis Pipeline: PIMBA consolidates data preprocessing, sequence alignment, taxonomic assignment, and statistical analyses into a single framework.
• End-to-end NGS Processing: PIMBA automates processing of large Next-Generation Sequencing datasets from raw sequence input to final output generation.

Scientific Applications:

• Biodiversity Monitoring: Assessing species diversity and abundance in ecosystems using DNA metabarcoding.
• Conservation Biology: Identifying rare or endangered species within habitats from metabarcoding data.
• Environmental Impact Studies: Evaluating the effects of human activities on biodiversity using NGS-based metabarcoding.

Methodology:

PIMBA integrates customizable databases with established reference databases and automates data processing and analysis steps from raw sequence data to final output generation.