piPipes

piPipes coordinates analysis of small RNA, RNA-seq, degradome sequencing, 7-methyl guanosine cap analysis of gene expression (CAGE), chromatin immunoprecipitation (ChIP) sequencing, and genomic DNA sequencing to support piRNA and related small RNA analyses and assessment of transposon expression in the metazoan germ line.


Key Features:

  • Pipeline Coordination: Provides coordinated pipelines tailored for small RNA, RNA-seq, degradome sequencing, CAGE, ChIP-seq, and genomic DNA sequencing data.
  • Standardization: Applies standardized computational methods for piRNA research to improve reproducibility and comparability across studies.
  • Data Visualization and Reporting: Produces publication-ready figures and tables from processed sequencing data.
  • Implementation Languages: Implemented using Bash, C++, Python, Perl, and R.

Scientific Applications:

  • piRNA and transposon repression studies: Enables analysis of piRNAs (23–36 nucleotides) that repress transposon expression in the metazoan germ line and contribute to genomic stability.

Methodology:

Pipelines are implemented using Bash, C++, Python, Perl, and R.

Topics

Details

Tool Type:
command-line tool
Operating Systems:
Linux
Programming Languages:
R, C, Python, Shell, C++, Perl
Added:
8/3/2017
Last Updated:
11/25/2024

Operations

Publications

Han BW, Wang W, Zamore PD, Weng Z. piPipes: a set of pipelines for piRNA and transposon analysis via small RNA-seq, RNA-seq, degradome- and CAGE-seq, ChIP-seq and genomic DNA sequencing. Bioinformatics. 2014;31(4):593-595. doi:10.1093/bioinformatics/btu647. PMID:25342065. PMCID:PMC4325541.

Documentation

Links