PitViper

PitViper integrates gold-standard methods for comparative meta-analysis and annotation of functional genomics screening data from diverse technologies.

Key Features:

• End-to-end automated pipeline: Provides reproducible bioinformatics analysis for functional screening data.
• Flexible and interactive framework: Enables data analysis and reanalysis from diverse functional screening methods.
• Reliable element-specific analysis: Delivers results tailored to the type of elements being screened, including genes, non-coding regions, and genomic DNA elements.
• Comprehensive data integration: Integrates diverse array of functional genomics screening outputs into a cohesive framework.

Scientific Applications:

• shRNA screening analysis: Analyzes functional screening data using single or pooled shRNA targeting genes or non-coding regions.
• CRISPR-Cas screening analysis: Processes sgRNA-based functional screening data targeting genes or non-coding regions.
• CRISPRi screening analysis: Identifies potential super-enhancer liabilities through genome-wide CRISPR-mediated interference screening.
• CRISPRa screening analysis: Analyzes CRISPR-mediated activation of targeted genes or genomic DNA elements.
• Comparative meta-analysis: Enables integration and comparison across diverse functional genomics screening datasets.

Methodology:

PitViper integrates gold-standard methods for functional screening analysis in an end-to-end automated and reproducible pipeline.