PlaASDB

PlaASDB catalogs alternative splicing (AS) events in Arabidopsis thaliana and Oryza sativa under biotic and abiotic stresses to enable comparative analyses of stress-responsive splicing and associated gene expression changes.

Key Features:

• Extensive Data Collection: Integration of 3,255 publicly available RNA-seq datasets from Arabidopsis thaliana and Oryza sativa covering diverse stress conditions.
• Alternative Splicing Event Detection: Computational detection of AS events and identification of differentially spliced genes (DSGs) with correlation to gene expression data.
• Comparative Analysis Capabilities: Cross-species comparison of AS patterns between Arabidopsis and rice under abiotic and biotic stress, revealing conservation of AS patterns relative to gene expression changes.
• Independent Roles of AS and Gene Expression: Characterization of limited overlap between DSGs and differentially expressed genes (DEGs) under stress, supporting independent regulatory roles.

Scientific Applications:

• Regulatory Mechanism Studies: Investigation of AS-mediated regulation of stress responses in plants at the transcriptomic level.
• Comparative Transcriptomics: Large-scale comparison of splicing patterns and gene expression between Arabidopsis thaliana and Oryza sativa under biotic and abiotic stresses.
• Crop Stress Research: Informing studies aimed at understanding and potentially improving stress resilience in crop species through AS analysis.

Methodology:

Collection of publicly available RNA-seq datasets followed by computational analyses to detect alternative splicing events, identify differentially spliced genes (DSGs), and assess differential gene expression (DEGs).