plant-rlk-rlp

plant-rlk-rlp performs orthology and synteny analysis of receptor-like kinases (RLK) and receptor-like proteins (RLP) across seven legume species to elucidate their evolutionary relationships and chromosomal distribution.

Key Features:

• Orthology Analysis: Evaluates homology relations among RLKs and RLPs across seven legume species and identifies orthologous clusters with 66–90% of RLKs and 83–88% of RLPs per species classified in these clusters.
• Synteny Analysis: Detects RLKs and RLPs within syntenic blocks, reporting 75–98% of these proteins present in syntenic regions and noting high chromosomal similarity between Phaseolus vulgaris and Vigna unguiculata (divergence ≈ 8 million years ago).
• Comparative Genomics: Maps RLK and RLP genes in synteny blocks to assess evolutionary conservation across legumes and identifies Phaseolus vulgaris as an appropriate anchor species for comparative genomic studies.
• Resistance Gene Identification: Identifies seven experimentally validated resistance RLK/RLP genes present in syntenic blocks, including FLS2, BIR2, ERECTA, IOS1, AtSERK1 (Arabidopsis), SLSERK1 (Solanum lycopersicum), and notes LYM1-RLP as the only gene shared with Glycine max.

Scientific Applications:

• Evolutionary analysis of RLK/RLP families: Elucidates dynamic evolution and orthologous relationships of RLKs and RLPs across legume species.
• Comparative genomics and anchoring: Enables comparative genomic analyses using Phaseolus vulgaris as an anchor to identify conserved chromosomal segments among legumes.
• Resistance gene conservation and stress biology: Identifies conserved resistance RLK/RLPs in syntenic blocks to inform studies of biotic and abiotic stress response mechanisms in legumes.

Methodology:

Performs orthology analysis and synteny analysis on genomic data to identify conserved clusters and chromosomal segments of RLK and RLP genes across multiple legume species.