PlantCV v2

PlantCV v2 performs image-based plant phenotyping using computer vision and image analysis techniques to quantify morphological, morphometric, and temporal traits.


Key Features:

  • Modular Design: Structured as modular components for reuse and composition of analysis steps.
  • Image Processing and Normalization Tools: Provides image processing and normalization functionalities to handle diverse imaging conditions and ensure consistent phenotype measurements.
  • Multi-Plant Image Analysis: Supports analysis of images containing multiple plants for high-throughput and field-condition datasets.
  • Leaf Segmentation: Implements leaf segmentation algorithms for precise delineation and measurement of leaf morphology.
  • Landmark Identification for Morphometrics: Includes landmark identification modules for morphometric extraction and shape analysis.
  • Machine Learning Modules: Integrates machine learning modules for pattern recognition, classification, and predictive modeling on plant images.

Scientific Applications:

  • Temporal Phenotyping: Enables non-destructive, high-resolution temporal measurement of plant phenotypes.
  • Morphological and Morphometric Studies: Supports morphological assessments and morphometric analysis using leaf segmentation and landmark identification.
  • High-Throughput and Field Phenotyping: Applicable to high-throughput phenotyping and analysis of images collected under field or multi-plant conditions.
  • Breeding and Trait Analysis: Facilitates complex trait analysis in breeding programs through quantitative image-derived traits.
  • Plant Biology, Agronomy, and Ecology Research: Used for trait quantification in research areas including plant biology, agronomy, and ecology.

Methodology:

Employs computer vision techniques including image processing, normalization, leaf segmentation, landmark identification for morphometrics, and machine learning-based classification and prediction applied to single- and multi-plant images within a modular component framework.

Topics

Details

Tool Type:
library
Operating Systems:
Linux, Windows, Mac
Programming Languages:
Python
Added:
7/16/2018
Last Updated:
12/10/2018

Operations

Publications

Gehan MA, Fahlgren N, Abbasi A, Berry JC, Callen ST, Chavez L, Doust AN, Feldman MJ, Gilbert KB, Hodge JG, Hoyer JS, Lin A, Liu S, Lizárraga C, Lorence A, Miller M, Platon E, Tessman M, Sax T. PlantCV v2: Image analysis software for high-throughput plant phenotyping. PeerJ. 2017;5:e4088. doi:10.7717/peerj.4088. PMID:29209576. PMCID:PMC5713628.

PMID: 29209576
PMCID: PMC5713628
Funding: - US National Science Foundation: DBI-1156581, IIA-1355406, IIA-1430427, IIA-1430428, IOS-1202682, MCB-1330562 - US Department of Energy: DE-AR0000594, DE-SC0014395 - US Department of Agriculture: 2016-67009-25639, MOW-2012-01361

Documentation