PlantMWpIDB

PlantMWpIDB provides molecular weight and isoelectric point (pI) data for proteins from 342 plant species to support proteomic analyses and interpretation of protein function and subcellular localization.

Key Features:

• Extensive Data Coverage: Contains molecular weight and pI data for proteins across 342 plant species.
• Precomputed Molecular Properties: Calculates and stores molecular weight and isoelectric point for each protein sequence.
• Virtual 2D Proteome Map: Provides virtual 2D proteome maps per species representing protein distributions by molecular weight and pI.
• Experimental Compatibility: Supports interpretation of 2D gel electrophoresis, liquid chromatography-mass spectrometry, and X-ray protein crystallography results.
• Sequence Query Support: Supports querying by protein sequence to relate sequence data to computed molecular properties.
• Functional and Localization Inference: Enables inference of subcellular localization and functional roles from molecular weight and pI patterns.

Scientific Applications:

• Proteomics Analysis: Aids interpretation of proteomic experiments such as 2D gels and liquid chromatography-mass spectrometry by supplying molecular weight and pI references.
• Protein Function and Localization Studies: Supports studies linking physicochemical properties of proteins to function and subcellular localization.
• Biotechnology and Functional Genomics: Supports hypothesis generation and development of biotechnological applications through comparative proteome analyses.
• Evolutionary and Comparative Biology: Enables comparative analyses of proteome diversity across plant species for evolutionary and functional genomics studies.

Methodology:

Calculation of molecular weight and isoelectric point for plant protein sequences and generation of virtual 2D proteome maps, with analysis of molecular weight and pI data to infer subcellular localization and functional roles.