PlantNATsDB

PlantNATsDB catalogs approximately 2 million natural antisense transcript (NAT) pairs across 69 plant species to support analysis of NAT-mediated gene regulation using integrated Gene Ontology annotations and small RNA sequencing data.

Key Features:

• Extensive Database Coverage: Contains approximately 2 million NAT pairs across 69 plant species.
• Integration of Diverse Data Sources: Integrates Gene Ontology (GO) annotations and high-throughput small RNA sequencing data linked to NAT pairs.
• Graphical Network Browser: Provides an integrated graphical network browser that represents interactions and networks formed by NATs.
• Gene Set Analysis Module: Implements a Gene Set Analysis module that uses GO annotations to identify statistically overrepresented GO categories within NAT networks.

Scientific Applications:

• Gene regulation analysis: Investigating regulatory roles of NATs in plant gene expression.
• Developmental biology: Studying NAT involvement in plant development processes.
• Stress response research: Examining NAT roles in plant stress responses using integrated small RNA sequencing data and GO annotations.
• Disease mechanism studies: Exploring potential contributions of NATs to plant disease mechanisms.

Methodology:

Aggregation and annotation of NAT data from multiple sources; incorporation of Gene Ontology (GO) annotations and high-throughput small RNA sequencing data; GO-based gene set overrepresentation analysis within NAT networks; network visualization via an integrated graphical network browser.